Detection of Candida albicans by Nested PCR
نویسندگان
چکیده
منابع مشابه
Detection of Candida albicans mRNA from formalin-fixed, paraffin-embedded mouse tissues by nested reverse transcription-PCR.
Histopathology archives represent a vast source of infectious disease specimens that can be used to elucidate important disease processes. In this report, we describe a method to detect Candida albicans gene expression from infected, formalin-fixed, paraffin-embedded mouse tissue. By use of glass beads to break open the fungal cells and proteinase K treatment, RNA was extracted routinely from t...
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The feasibility of detecting Candida albicans mRNA in formalin-fixed paraffin-embedded archival human histopathology specimens by reverse transcription-PCR (RT-PCR) was investigated. RT with gene-specific primers was used to detect five single-copy C. albicans gene transcripts, including those of two housekeeping genes, in oral candidiasis samples up to 8 years of age.
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Introduction: Malaria is one of the most important parasitic diseases in tropical and sub-tropical areas of the world and kills a large number of People annually. Diagnosis of the disease is preformed by microscopic and molecular methods. This study has been designed for detecting of latent/sub-patent infection caused by plasmodium vivax in individuals with history of vivax malaria without an...
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ژورنال
عنوان ژورنال: Journal of the Japanese Association for Infectious Diseases
سال: 1994
ISSN: 1884-569X,0387-5911
DOI: 10.11150/kansenshogakuzasshi1970.68.1465